Staphylococcus aureusAdhesion via Spa, ClfA, and SdrCDE to Immobilized Platelets Demonstrates Shear-Dependent Behavior
نویسندگان
چکیده
منابع مشابه
Staphylococcus aureus adhesion via Spa, ClfA, and SdrCDE to immobilized platelets demonstrates shear-dependent behavior.
OBJECTIVE The objective of this study is to delineate the molecular mechanisms responsible for Staphylococcus aureus-platelet adhesion as a function of physiologically relevant wall shear stresses. METHODS AND RESULTS A parallel plate flow chamber was used to quantify adhesion of wild-type, Spa-, ClfA- and SdrCDE- strains to immobilized platelet layers. In the absence of plasma, adhesion incr...
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BACKGROUND Methicillin-resistant Staphylococcus aureus (MRSA) is an increasingly prevalent pathogen capable of causing severe vascular infections. The goal of this work was to investigate the role of shear stress in early adhesion events. METHODS Human umbilical vein endothelial cells (HUVEC) were exposed to MRSA for 15-60 minutes and shear stresses of 0-1.2 Pa in a parallel plate flow chambe...
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OBJECTIVE Staphylococcus aureus is the most frequent causative organism of infective endocarditis (IE) and is characterized by thrombus formation on a cardiac valve that can embolize to a distant site. Previously, we showed that S. aureus clumping factor A (ClfA) and fibronectin-binding protein A (FnBPA) can stimulate rapid platelet aggregation. METHODS AND RESULTS In this study we investigat...
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متن کاملPlatelets and shear stress.
C LINICAL BLEEDING or thrombosis results from a disturbance in the balance between a complex network of procoagulant and anticoagulant factors. This network involves three primary interactions, first described by the eminent pathologist Rudolph Virchow during the previous century, between blood (soluble and cellular constituents), the blood vessel (including fixed and dynamic responses), and bl...
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ژورنال
عنوان ژورنال: Arteriosclerosis, Thrombosis, and Vascular Biology
سال: 2006
ISSN: 1079-5642,1524-4636
DOI: 10.1161/01.atv.0000237606.90253.94